Doświadczenie wykazało, że fungicydy zastosowane w ustalonym wariancie badawczym nie miały istotnego wpływu na podstawowe parametry składu chemicznego ziarna, takie jak zawartość białka czy skrobi. We wszystkich badanych wariantach najczęściej izolowany­mi rodzajami grzybów były Alternaria oraz Epicoccum. Grzyby z rodzaju Fusarium izolowano najliczniej z prób kontrolnych, a następniez wariantów opryskiwanych azoksystrobiną i fenpropimorfem. Zauważono, że stosowanie obniżonych dawek większości fungicydów nie zawsze sprzyjało zwiększonej częstotliwości występowania Fusarium spp. w porównaniu z dawkami zalecanymi. Analiza zawartości my­kotoksyn wykazała, że najwyższe stężenie deoksyniwalenolu (DON) występowało w próbach ziarna chronionego tebukonazolem i azok­systrobiną, natomiast najwyższe poziomy zearalenonu (ZEA) odnotowano w próbach traktowanych tiofanatem metylowym, tebukonazo­lem i fenpropimorfem – przy czym zjawisko to obserwowano wyłącznie w wariantach, w których zastosowano połowę dawki zalecanej.

The analysis showed that the application of fungicides did not have a significant effect on the basic chemical composition of the grain, such as protein or starch content. In all tested variants, the most frequently isolated were fungi from genera Alternaria and Epicoccum, confirming their dominant role in the grain microbiome. Fungi from the genus Fusarium were most isolated from control samples, fol­lowed by those treated with azoxystrobin and fenpropimorph. It was observed that the use of reduced doses of most fungicides has not always promoted a higher frequency of Fusarium spp. occurrence compared to recommended doses. Mycotoxin analysis revealed that the highest concentration of deoxynivalenol (DON) was detected in grain samples treated with tebuconazole and azoxystrobin, while the highest levels of zearalenone (ZEA) were recorded in samples treated with thiophanate-methyl, tebuconazole and fenpropimorph – although this phenomenon was observed exclusively in variants where half of the recommended dose had been applied.

Almoujahed M.B., Apolo-Apolo O.E., Morellos A., Pantazi X.E., Kazlauskas M., Kriaučiūnienė Z., Šarauskis E., Mouazen A.M. 2025. Cost-benefit evaluation of preventive site-specific fungicide spraying for fusarium head blight in wheat. Computers and Electronics in Agriculture 238: 110836. DOI: 10.1016/j.compag.2025.110836

Amarasinghe C.C., Tamburic-Ilincic L., Gilbert J., Brûlé-Babel A.L., Fernando W.G.D. 2013. Evaluation of different fungicides for control of fusarium head blight in wheat inoculated with 3ADON and 15ADON chemotypes of Fusarium graminearum in Canada. Canadian Journal of Plant Pathology 35 (2): 200–208. DOI: 10.1080/07060661.2013.773942

Blandino M., Reyneri A. 2009. Effect of fungicide and foliar fertilizer application to winter wheat at anthesis on flag leaf senes­cence, grain yield, flour bread-making quality and DON contamination. European Journal of Agronomy 30 (4): 275–282. DOI: 10.1016/j.eja.2008.12.005

Bolanos-Carriel C., Wegulo S.N., Baenziger P.S., Funnell-Harris D., Hallen-Adams H.E., Eskridge K.M. 2020. Effects of fungicide chemical class, fungicide application timing, and environment on Fusarium head blight in winter wheat. European Journal of Plant Pathology 158 (3): 667–679. DOI: 10.1007/s10658-020-02109-3

Booth C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England, 237 ss.

Butkutė B., Mankevičienė A., Gaurilčikienė I. 2008. A comparative study of strobilurin and triazole treatments in relation to the incidence of Fusarium head blight in winter wheat, grain quality and safety. Cereal Research Communications 36 (6): 671–675. DOI: 10.1556/CRC.36.2008.Suppl.B.58

Cromey M.G., Lauren D.R., Parkes R.A., Sinclair K.I., Shorter S.C., Wallace A.R. 2001. Control of Fusarium head blight of wheat with fungicides. Australasian Plant Pathology 30 (4): 301–308. DOI: 10.1071/AP01065

Elsherbini A., Abdel Halim K., Beltagy R., Amine H.M. 2024. Collateral effects of some fungicides commonly used on wheat plants. Journal of Sustainable Agricultural and Environmental Sciences 3 (5): 1–13. DOI: 10.21608/jsaes.2024.315318.1099

Eurostat 2024. Key figures on the European food chain – 2024 edition [online]. https://ec.europa.eu/eurostat/web/products-key-figures/w/ks-01-24-000 [dostęp: 11.09.2025].

FRAC (Fungicide Resistance Action Committee) 2025. [online]. https://www.frac.info/fungicide-resistance-management/by-fun­gicide-common-name/#open-tour [dostęp: 11.09.2025].

Gaurilčikienė I., Butkutė B., Mankevičienė A., Paplauskienė V. 2010. A multi-aspect comparative investigation on the use of stro­bilurin and triazole-based fungicides for winter wheat disease control. s. 69–94. W: Fungicides (O. Carisse, red.). BoD – Books on Demand, Hamburg, 500 ss.

Groß J., Gentsch N., Boy J., Heuermann D., Schweneker D., Feuerstein U., Brunner J., von Wiren N., Guggenberger G., Bauer B. 2023. Influence of small-scale spatial variability of soil properties on yield formation of winter wheat. Plant and Soil 493 (1): 79–97. DOI: 10.1007/s11104-023-06212-2

Haidukowski M., Pascale M., Perrone G., Pancaldi D., Campagna C., Visconti A. 2005. Effect of fungicides on the development of Fusarium head blight, yield and deoxynivalenol accumulation in wheat inoculated under field conditions with Fusarium gra­minearum and Fusarium culmorum. Journal of the Science of Food and Agriculture 85 (2): 191–198. DOI: 10.1002/jsfa.1965

Kayim M., Nawaz H., Alsalmo A. 2022. Fungal diseases of wheat. W: Wheat-Recent Advances (M.R. Ansari, red.). IntechOpen, London, UK.

Kwaśna H., Chełkowski J., Zajkowski P. 1991. Flora Polska. Grzyby (Mycota). Tom XXII. Sierpik (Fusarium). Instytut Botaniki, Polska Akademia Nauk, Warszawa–Kraków, 136 ss.

Li C., Fan S., Zhang Y., Zhang X., Luo J., Liu C. 2022. Toxicity, bioactivity of triazole fungicide metconazole and its effect on mycotoxin production by Fusarium verticillioides: New perspective from an enantiomeric level. Science of The Total Environ­ment 828: 154432. DOI: 10.1016/j.scitotenv.2022.154432

Lucas J.A., Hawkins N.J., Fraaije B.A. 2015. The evolution of fungicide resistance. Advances in Applied Microbiology 90: 29–92. DOI: 10.1016/bs.aambs.2014.09.001

Matysiak K., Siatkowski I., Kierzek R., Kowalska J., Krawczyk R. 2020. Effect of foliar applied acetylsalicilic acid on wheat (Triti­cum aestivum L.) under field conditions. Agronomy 10 (12): 1918. DOI: 10.3390/agronomy10121918

Mercer P.C., Ruddock A. 2005. Disease management of winter wheat with reduced doses of fungicides in Northern Ireland. Crop Protection 24 (3): 221–228. DOI: 10.1016/j.cropro.2004.07.009

Mesterházy Á., Bartók T., Lamper C. 2003. Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the ef­ficacy of fungicides for control of Fusarium head blight. Plant Disease 87 (9): 1107–1115. DOI: 10.1094/PDIS.2003.87.9.1107

Metcalfe R.J., Shaw M.W., Russell P.E. 2000. The effect of dose and mobility on the strength of selection for DMI fungicide resis­tance in inoculated field experiments. Plant Pathology 49 (5): 546–557. DOI: 10.1046/j.1365-3059.2000.00486.x

Paul P.A., Bradley C.A., Madden L.V., Dalla Lana F., Bergstrom G.C., Dill-Macky R., Esker P.D., Wise K.A., McMullen M., Grybauskas A., Kirk W.W., Milus E., Ruden K. 2018. Meta-analysis of the effects of QoI and DMI fungicide combinations on Fusarium head blight and deoxynivalenol in wheat. Plant Disease 102 (12): 2602–2615. DOI: 10.1094/PDIS-02-18-0211-RE

Paul P.A., Lipps P.E., Hershman D.E., McMullen M.P., Draper M.A., Madden L.V. 2008. Efficacy of triazole-based fungicides for Fusarium head blight and deoxynivalenol control in wheat: a multivariate meta-analysis. Phytopathology 98 (9): 999–1011. DOI: 10.1094/PHYTO-98-9-0999

Simpson D.R., Weston G.E., Turner J.A., Jennings P., Nicholson P. 2001. Differential control of head blight pathogens of wheat by fungicides and consequences for mycotoxin contamination of grain. European Journal of Plant Pathology 107 (4): 421–431. DOI: 10.1023/A:1011225817707

Singh N., Virdi A.S., Katyal M., Kaur A., Kaur D., Ahlawat A.K., Singh A.M., Sharma R.K. 2021. Evaluation of heat stress through delayed sowing on physicochemical and functional characteristics of grains, whole meals and flours of India wheat. Food Chemistry 344: 128725. DOI: 10.1016/j.foodchem.2020.128725

Somma S., Scarpino V., Quaranta F., Logrieco A., Reyneri A., Blandino M., Moretti A. 2022. Impact of fungicide application to control T-2 and HT-2 toxin contamination and related Fusarium sporotrichioides and F. langsethiae producing species in durum wheat. Crop Protection 159: 106020. DOI: 10.1016/j.cropro.2022.106020

Supronienė S., Mankevičienė A., Kadžienė G., Kačergius A., Feiza V., Feizienė D., Semaškienė R., Dabkevičius Z., Tamošiūnas K. 2012. The impact of tillage and fertilization on Fusarium infection and mycotoxin production in wheat grains. Žemdirbystė 99 (3): 265–272.

Van den Berg F., Paveley N.D., Van den Bosch F. 2016. Dose and number of applications that maximize fungicide effective life exemplified by Zymoseptoria tritici on wheat - a model analysis. Plant Pathology 65 (8): 1380–1389. DOI: 10.1111/ppa.12558

Wachowska U., Waśkiewicz A., Jędryczka M. 2017. Using a protective treatment to reduce Fusarium pathogens and mycotoxins contaminating winter wheat grain. Polish Journal of Environmental Studies 26 (5): 2277–2286. DOI: 10.15244/pjoes/67747

Wegulo S.N., Zwingman M.V., Breathnach J.A., Baenziger P.S. 2011. Economic returns from fungicide application to control foliar fungal diseases in winter wheat. Crop Protection 30 (6): 685–692. DOI: 10.1016/j.cropro.2011.02.002

Wilkinson A.P., Ward C.M., Morgan M.R.A. 1992. Immunological analysis of mycotoxins. s. 185–225. W: Plant Toxin Analysis (H.F. Linskens, J.F. Jackson, red.). Springer Berlin, Heidelberg, 390 ss. DOI: 10.1007/978-3-662-02783-7

Wiwart M., Perkowski J., Budzyński W., Suchowilska E., Buśko M., Matysiak A. 2011. Concentrations of ergosterol and trichothe­cenes in the grains of three Triticum species. Czech Journal of Food Sciences 29 (4): 430–440. DOI: 10.17221/229/2010-CJFS

Wyczling D., Pańka D., Lenc L., Sadowski C. 2005. Wpływ ochrony fungicydami na zdrowotność i plon pszenicy ozimej. Acta Agrobotanica 58 (2): 287–306.

Zalecenia ochrony roślin na lata 2018/2019. Opracowanie zbiorowe. Instytut Ochrony Roślin – Państwowy Instytut Badawczy, Poznań, 359 ss.