Celem badania było określenie wpływu czynników biologicznych – mikroorganizmów (bakterii Pseudomonas spp. i drożdży Saccharo­myces cerevisiae) oraz tatarakowego olejku eterycznego (Acorus calamus L.), stosowanych łącznie lub oddzielnie, na zachowanie się izofetamidu w sałacie (Lactuca sativa L.). Sałatę poddano działaniu fungicydu zawierającego izofetamid (3,6 mg/roślina), a po upływie2 godzin przeprowadzono zabiegi: wariant 1 – olejek tatarakowy (12%), wariant 2 – olejek tatarakowy (12%) + Pseudomonas (106 jtk/ml), wariant 3 – olejek tatarakowy (12%) + Saccharomyces cerevisiae (106 jtk/ml). Grupę kontrolną stanowiły rośliny sałaty poddane jedynie działaniu izofetamidu. Po aplikacji izofetamidu, oznaczono jego stężenie za pomocą chromatografii cieczowej sprężonej z tandemową spektrometrią mas (LC-MS/MS) i wyznaczono dynamiki zanikania tego związku w liściach sałaty. Analiza wykładniczych modeli rozkładu potwierdziła wpływ czynników biologicznych na tempo degradacji izofetamidu, przy wysokim dopasowaniu danych (R² = 0,8629–0,9749). W kontroli czas połowicznego rozkładu izofetamidu wyniósł DT50 = 13,2 dnia. Najszybszy rozkład obserwowano w wariancie łączącym olejek tatarakowy z Pseudomonas (DT50 = 7,4 dnia). Wyniki badań wskazują, że czynniki biologiczne stosowane łącznie z izofetamidem wpływają na zachowanie izofetamidu w liściach sałaty.

The aim of the study was to determine the effect of biological factors – microorganisms (Pseudomonas spp. and Saccharomyces cere­visiae) and calamus essential oil (Acorus calamus L.), used combined or separately, on the behavior of isofetamid in lettuce (Lactucasativa L.). The lettuce was exposed to fungicide containing isofetamid (3.6 mg/plant), and after 2 hours, the following treatments were carried out: variant 1 – calamus oil (12%), variant 2 – calamus oil (12%) + Pseudomonas (106 CFU/ml), variant 3 – calamus oil (12%) + Saccharomyces cerevisiae (106 CFU/ml). The control group included lettuce plants treated only with isofetamid. After the application of isofetamid, its concentration was determined by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) the dynamics of the compound’s loss in lettuce leaves were determined. Analysis of exponential degradation models confirmed the influ­ence of biological factors on the rate of isofetamid degradation, with a high data fit (R² = 0.8629–0.9749). In the control group, the half-life of isofetamid was DT50 = 13.2 days. The fastest degradation was observed in the variant combining calamus oil with Pseudomonas(DT50 = 7.4 days). The results of the study indicate that biological agents used in combination with isofetamid affect the behavior of the isofetamid in lettuce leaves.

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