Celem badań było określenie profilu fitochemicznego ekstraktu z liści firletki chalcedońskiej (Silene chalcedonica) oraz ocena jego aktywności supresyjnej wobec osobników drugiego stadium młodocianego (J2s) nicienia Meloidogyne hapla w kontrolowanych wa­runkach komory fitotronowej. Analiza fitochemiczna wykazała obecność 20-hydroksyekdyzonu (20E), którego zawartość wynosiła0,19 mg/g suchej masy, przy jednocześnie niskiej zawartości polifenoli ogółem oraz niskiej aktywności antyoksydacyjnej. Zastosowany preparat roślinny istotnie ograniczał tworzenie wyrośli korzeniowych oraz liczbę larw zasiedlających tkanki roślin żywicielskich, szcze­gólnie w niższej spośród badanych temperatur, tj. 15°C w porównaniu z 20°C, wskazując na istotny potencjał supresyjny wobec nicieni. Uzyskane wyniki sugerują, że ekstrakty z liści S. chalcedonica mogą stanowić obiecujący, przyjazny dla środowiska element strategii zrów­noważonego ograniczania populacji guzaków (Meloidogyne spp.).

The aim of this study was to determine the phytochemical profile of Silene chalcedonica leaf extract and to evaluate its nematode­-suppressive activity against second-stage juveniles (J2s) of Meloidogyne hapla under controlled growth chamber conditions. Phyto­chemical analysis revealed the presence of 20-hydroxyecdysone (20E) at a concentration of 0.19 mg/g dry weight (DW), accompanied by low total phenolic content and low antioxidant activity. The botanical preparation significantly reduced root gall formation and the number of juveniles established within host plant tissues, particularly at the lower tested temperature of 15°C compared with 20°C,indicating significant suppressive activity against nematodes. The obtained results suggest that S. chalcedonica leaf extracts may representa promising, environmentally friendly component of strategies for the sustainable management of root-knot nematodes(Meloidogyne spp.).

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