Progress in Plant Protection

Sekwencjonowanie następnej generacji (NGS) jako uniwersalna metoda wykrywania i różnicowania wirusów roślinnych
Next generation sequencing (NGS) as a multipurpose method for detection and differentiation of plant viruses

Agata Kaczmarek, e-mail: a.kaczmarek@ihar.edu.pl

Instytut Hodowli i Aklimatyzacji Roślin – Państwowy Instytut Badawczy, Radzików, 05-870 Błonie, Polska

Krzysztof Treder, e-mail: k.treder@ihar.edu.pl

Instytut Hodowli i Aklimatyzacji Roślin – Państwowy Instytut Badawczy, Radzików, 05-870 Błonie, Polska
Streszczenie

Niniejsza praca opisuje wykorzystanie sekwencjonowania następnej generacji (NGS) w badaniach nad wirusami roślin. Pomimo iż, NGS nie jest jeszcze rutynowo stosowane, coraz częściej jest adaptowane w diagnostyce oraz genomice fitopatogenów. Techniki NGS umożliwiają jednoczesne wykrycie wielu wirusów obecnych w zakażonym materiale. Dzięki nim możliwe jest nie tylko stwierdzenie, jakie wirusy są obecne w jednej badanej próbie, ale również ich zróżnicowanie genetyczne. Jednoczesna identyfikacja wielu wirusów, możliwość wczesnego wykrywania ognisk choroby, śledzenie rozwoju epidemii oraz monitorowanie zmian genetycznych zachodzących w populacji patogenów wirusowych w trakcie rozwoju epidemii sprawiają, że NGS staje się uniwersalnym narzędziem badawczym umożliwiającym nie tylko detekcję, ale również zrozumienie mechanizmów molekularnych pozwalających wirusom adaptować się do zmian środowiskowych (genotypu rośliny – gospodarza, wektora, obecności innych patogenów).

 

This paper describes the application of next generation sequencing (NGS) in plant virus research. Although NGS has not been routinely used yet, it is increasingly adopted in diagnostics and genomics of phytopathogens. NGS technics enable the simultaneous detection of multiple viruses present in infected material. This makes it possible not only to determine which viruses are present in a single sample but also to determine their concentration and genetic diversity. The simultaneous identification of many viruses, the possibility of early detection of disease outbreaks as well as tracking and monitoring of epidemic development, make NGS a universal research tool that enables not only the detection but also the understanding of molecular mechanisms allowing viruses to adapt to environmental changes (host plant genotype, vector, presence of other pathogens).

Słowa kluczowe
diagnostyka; fitopatologia; NGS; sekwencjonowanie; wirusy roślin; diagnostics; plant pathology; sequencing; plant viruses
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Progress in Plant Protection (2021) : 0-0
Data pierwszej publikacji on-line: 2021-10-08 11:06:47
http://dx.doi.org/10.14199/ppp-2021-029
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