Progress in Plant Protection

Ekspresja genów kodujących oksydazę polifenolową oraz amoniakoliazę L-fenyloalaniny pszenicy w odpowiedzi na żerowanie larw Oulema spp.
Expression of genes encoding polyphenol oxidase and phenylalanine ammonia-lyase in wheat in response to Oulema spp. larvae feeding

Beata Wielkopolan, e-mail: B.Wielkopolan@iorpib.poznan.pl

Instytut Ochrony Roślin – Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań, Polska
Streszczenie

Skrzypionki należą do ważnych gospodarczo agrofagów zbóż, ponieważ ich żerowanie, szczególnie stadium larwalnego, może znacznie zmniejszyć jakość oraz ilość uzyskanego plonu. Celem pracy było sprawdzenie ekspresji genów PPO oraz PAL biorących udział w produkcji metabolitów wtórnych, w odpowiedzi na żerowanie larw skrzypionek ze zredukowanym lub niezredukowanym komponentem bakteryjnym. Wykazano, że bakterie związane z larwami skrzypionek tłumią odpowiedź pszenicy na korzyść gospodarza – owada. Każda z trzech odmian pszenicy wykształciła unikalny mechanizm obronny na żerowanie larw skrzypionek (między innymi uszkodzenie mechaniczne czy uszkodzenie powstałe w wyniku żerowania owada). Odmiana Arkadia znacznie szybciej oraz silniej reagowała na traktowanie w porównaniu do dwóch pozostałych odmian pszenicy. Zrozumienie ekspresji genów zaangażowanych w obronę roślin przeciwko roślinożernym szkodnikom jest bardzo ważne z ekologicznego punktu widzenia i ma duży potencjał do wykorzystania w ochronie roślin.

 

Cereal leaf beetle (CLB, Oulema spp.) are economically important pests of cereals, because their feeding, especially at the larval stage may significantly reduce the quality and quantity of the obtained crop. The aim of the study was explanation the expression level of PPO and PAL genes involved in the secondary metabolites production in response to feeding of CLB larvae with a reduced or not reduced bacterial component. It was indicated that bacteria associated with CLB larvae supressed wheat response to CLB feeding to the benefit of insect host. Each of the three wheat varieties developed a unique defence mechanism against tested stress factors (including mechanical damage or damage caused by larvae feeding). The Arkadia variety reacted much faster and stronger to treatments in comparison to the other two wheat varieties. Understanding of the expression of genes involved in the plant defence response against herbivory pests is very important from an ecological point of view and has a great potential for use in plant protection.

Słowa kluczowe
Oulema spp.; pszenica; odpowiedź obronna; bakterie; metabolity wtórne; PPO; PAL; wheat; defense response; bacteria; secondary metabolites
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Progress in Plant Protection (2021) : 0-0
Data pierwszej publikacji on-line: 2021-10-28 15:10:42
http://dx.doi.org/10.14199/ppp-2021-033
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